Blood-Clot
发表于 2025-3-23 13:34:45
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Hectic
发表于 2025-3-23 17:29:41
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心胸开阔
发表于 2025-3-23 21:00:12
Full-Length Single-Cell RNA Sequencing with Smart-seq2 characterization of rare cell types as well as the detailed analysis of the stochastic nature of gene expression. A large number of methods have been developed, varying in their throughput, sensitivity, and scalability. A major distinction is whether they profile only 5′- or 3′-terminal part of the
Hemoptysis
发表于 2025-3-24 00:11:28
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拒绝
发表于 2025-3-24 05:27:33
Single-Cell RNA-Seq by Multiple Annealing and Tailing-Based Quantitative Single-Cell RNA-Seq (MATQ-S heterogeneity in the transcriptome, high accuracy and sensitivity are still desired for single-cell RNA-seq. We describe here multiple annealing and dC-tailing-based quantitative single-cell RNA-seq (MATQ-seq) with ~90% capture efficiency. In addition, MATQ-seq is a total RNA assay allowing for det
expire
发表于 2025-3-24 07:17:15
Single-Cell RNA Sequencing with Drop-Seqcapture microparticles and cells are coconfined through a microfluidic device within the droplets where they undergo cell lysis and RNA hybridiztion. After breaking the droplets and pooling the hybridized particles, reverse transcription, PCR, and sequencing in single reactions allow to generate dat
受辱
发表于 2025-3-24 14:00:40
Chromium 10× Single-Cell 3′ mRNA Sequencing of Tumor-Infiltrating Lymphocytesin parallel. Chromium10× by 10× Genomics is an emulsion-based device that enables to compartmentalize single cells along with sets of uniquely barcoded primers and reverse transcription reagents into nanoscale droplets that are used as reaction chambers to generate barcoded full-length cDNA from sin
呼吸
发表于 2025-3-24 15:02:55
Seq-Well: A Sample-Efficient, Portable Picowell Platform for Massively Parallel Single-Cell RNA Sequlinical samples. In Seq-Well, uniquely barcoded mRNA capture beads and cells are co-confined in picowells that are sealed using a semipermeable membrane, enabling efficient cell lysis and mRNA capture. The beads are subsequently removed and processed in parallel for sequencing, with each transcript’
马赛克
发表于 2025-3-24 21:44:44
Book 2019ailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and single cell live imaging. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respectiv
Cabinet
发表于 2025-3-24 23:42:43
Book 2019e topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Single Cell Methods: Sequencing and Proteomics. aims to make each experiment easily reproducible in every lab.. .