Cabinet
发表于 2025-3-28 15:55:39
Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domainlay technology. The superbinders are stable and can be easily produced in . in large quantities. The strategy presented here may also be applied to other protein domains to generate domain variants with markedly enhanced affinities for a specific post-translational modification.
不合
发表于 2025-3-28 22:27:16
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BANAL
发表于 2025-3-29 02:10:51
978-1-4939-8295-0Springer Science+Business Media LLC 2017
内向者
发表于 2025-3-29 03:59:35
SH2 Domains978-1-4939-6762-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
Ballad
发表于 2025-3-29 11:14:57
Kazuya Machida,Bernard A. LiuIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
草率女
发表于 2025-3-29 14:03:14
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DENT
发表于 2025-3-29 16:47:03
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carbohydrate
发表于 2025-3-29 21:17:53
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矛盾
发表于 2025-3-30 01:56:10
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青春期
发表于 2025-3-30 05:45:48
Expression and Purification of SH2 Domains Using Baculovirus Expression Systeme insoluble in bacteria, baculovirus-insect cell expression systems can be an alternative to produce soluble and functionally active proteins. We describe a protocol for cloning and purification of GST-tagged SH2 domains using the Bac-to-Bac baculovirus expression system.