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Fanny Ott,Märit-Runa Jönsson,Kay Grünewald,Jan HellertPtsd429 发表于 2025-3-24 10:17:19
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Expression and Purification of RVFV Glycoprotein C for Structural Studies Using Baculovirus Expressn. In this chapter, we describe a simplified and scalable protocol facilitating the generation of high-quality, high-titer baculovirus virus for expression and purification of RVFV G., utilizing the baculovirus-mediated expression system in insect cells.Inflammation 发表于 2025-3-24 20:46:22
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Nucleotide Resolution Mapping of Rift Valley Fever Virus Nucleoprotein-Genome RNA Interactions,inking of direct protein-RNA interactions by UV irradiation. Following sample lysis, a selective isolation of N in complex with its RNA targets is achieved by immunoprecipitation. Then, N-RNA complexes are separated by SDS-PAGE, and after membrane transfer, RNA is isolated and subjected to library p