gimmick 发表于 2025-3-25 04:20:16
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Analysis of Global Conformational Transitions in Ribozymes,zymes contain one or more helical junctions, that act as key architectural elements in the structures. A combination of comparative gel electrophoresis and fluorescence resonance energy transfer (FRET) can be used to analyze the global conformation in solution, and to study the ion-induced folding pcushion 发表于 2025-3-25 15:40:30
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Selection In Vitro of Allosteric Ribozymes,these distinct functional classes may be combined to generate multipartite ribozymes in which the catalytic and ligand-binding elements act coordinately. In this chapter, we describe a procedure known as allosteric selection, in which an RNA catalyst in conjunction with a random sequence domain is s卧虎藏龙 发表于 2025-3-26 01:06:58
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Ribozyme Expression Systems,h level of production of ribozymes in vivo. The engineered tRNA.-driven ribozymes, based on computer-predicted secondary structure, were relatively stable and were transported to the cytoplasm, where they could be colocalized with their target RNA. The activity of the exported ribozymes was significFUSE 发表于 2025-3-26 15:24:47
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