涌出 发表于 2025-3-21 19:47:54
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Cloning and Detection of Genomic Retrozymes and Their circRNA Intermediateser retrotransposons, transcription of retrozymes in vivo leads to the formation and accumulation of circular RNAs, which can be readily detected by RNA blotting. In this chapter, we describe the procedures needed to carry out the cloning of genomic retrozymes, and to detect by northern blot their circular RNA retrotransposition intermediates.Complement 发表于 2025-3-22 02:23:07
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Book 2021 on troubleshooting and avoiding known pitfalls... Authoritative and cutting-edge, .Ribozymes: Methods and Protocols .aims to help in accelerating ribozyme research and inspiring others to develop new methods to study ribozyme structure and function..ALERT 发表于 2025-3-22 23:25:21
Conjugation as a Highly Sensitive Assay to Study Group II Intron Splicing In Vivomine the splicing competence of various Ll.LtrB mutants, whereas the splicing selection/conjugation assay was established to isolate splicing-proficient variants from a randomly generated bank of mutated introns.怒目而视 发表于 2025-3-23 02:05:32
Co-transcriptional Analysis of Self-Cleaving Ribozymes and Their Ligand Dependenceication or additional preparation steps, such as refolding procedures. Furthermore, because the transcripts are not denatured, this assay likely yields RNAs in conformations relevant to co-transcriptionally folded species in vivo.defile 发表于 2025-3-23 06:49:52
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