urinary-tract 发表于 2025-3-21 16:17:40
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Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemica modular design enables fast access to a large number of purine riboswitch derivatives with the modification of interest at any strand position. We exemplarily provide a detailed protocol for the preparation of adenosine deaminase (.) A-riboswitch variants with 2-aminopurine (AP) modifications at the 40-nmol scale.ACE-inhibitor 发表于 2025-3-22 02:45:45
Transcriptional Approaches to Riboswitch Studies,in biosynthesis and sulfur metabolism. The system can be utilized to facilitate biochemical studies of riboswitches in general, i.e., to simplify analysis of riboswitches that are not necessarily involved in transcriptional control.Fibrinogen 发表于 2025-3-22 07:51:06
,Monitoring RNA–Ligand Interactions Using Isothermal Titration Calorimetry,etail this method using the titration of an adenine-binding RNA with 2,6-diaminopurine (DAP) as a practical example. Our insights should be generally applicable to observing the interactions of a broad range of molecules with structured RNAs.MOAN 发表于 2025-3-22 09:10:45
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Predicting Riboswitch Regulation on a Genomic Scale, used to predict new examples of these structured RNAs. Since riboswitches generally function as .-regulatory elements, examining the genomic contexts of these hits is critical for evaluating their biological relevance. With these two sources of comparative support, it is possible to identify riboswpenance 发表于 2025-3-23 07:46:06
Enzymatic Ligation Strategies for the Preparation of Purine Riboswitches with Site-Specific Chemicact at the transcriptional or translational level and off- or on-regulate genes upon binding to their dedicated ligands. Biophysical studies on ligand-induced folding of these RNA domains in vitro contribute to understanding their regulation mechanisms in vivo. For such studies, in particular, for ap