Odious 发表于 2025-3-21 18:01:30
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Riboregulator Design and Analysis978-1-0716-2421-0Series ISSN 1064-3745 Series E-ISSN 1940-6029Genome 发表于 2025-3-22 02:05:20
James Chappell,Melissa K. TakahashiIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the expertsExpurgate 发表于 2025-3-22 06:47:09
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Design, Characterization, and Application of Targeted Gene Activation in Bacteria Using a Modular Cerest. Here, we describe a protocol for designing, characterizing, and applying the modular CRISPRa system for gene activation in .. We first focus on how to identify activating sites upstream of promoters and the cloning of the targeting sgRNA. We then describe how to perform a fluorescence experim