happiness
发表于 2025-3-28 17:47:04
Protein Engineering of Restriction Enzymes,icity is one of the scientific goals in studying these enzymes (.). This review focuses on protein engineering regarding Type II restriction enzymes. They comprise the vast majority of the about 3600 entities listed in REBASE today (.), although the number of those studied in detail is far smaller.
蔓藤图饰
发表于 2025-3-28 18:48:40
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defuse
发表于 2025-3-29 02:23:33
0933-1891 ucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known repre
Acupressure
发表于 2025-3-29 05:35:00
Book 2004gainst foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic seque
小平面
发表于 2025-3-29 08:48:33
Restriction-Modification Systems as Minimal Forms of Life, Restriction enzymes will cleave incoming DNA if it has not been modified by a cognate or another appropriate methyltransferase (Fig. 1B). Consequently, it is widely believed that restriction-modification systems have been maintained by bacteria because they serve to defend the cells from infection by viral, plasmid, and other foreign DNAs(.).
开始发作
发表于 2025-3-29 12:11:49
Restriction Endonucleases: Structure of the Conserved Catalytic Core and the Role of Metal Ions inthis Vol.) cleave specifically within or close to their recognition sites, and do not require ATP hydrolysis for their nucleolytic activity. DNA cleavage by these enzymes can result in DNA with either 5′ or 3′ overhangs or blunt ends.
装饰
发表于 2025-3-29 19:36:42
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被诅咒的人
发表于 2025-3-29 23:09:45
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SHRIK
发表于 2025-3-30 03:27:11
The Role of Water in the EcoRI-DNA Binding,TTC, with an association equilibrium constant K.∼10. M. and to a completely nonspecific sequence with K. ∼10.M.. A change of even a single base pair is sufficient to decrease the binding constant at least by 10., bringing it within a factor ∼10 or less of nonspecific binding (.).
Foolproof
发表于 2025-3-30 06:00:55
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