烹饪
发表于 2025-3-21 18:17:58
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Pillory
发表于 2025-3-21 21:31:25
Lambda Red Recombineering of Bacteriophage in the Lysogenic State,genome of a lysogenized host with a prophage compatible with bacteriophage lambda. Linear DNA is used as the recombination substrate and antibiotic resistance is used as the basis for selection of recombinants. The method enables the genetic manipulation of a prophage in 3–5 days.
比赛用背带
发表于 2025-3-22 03:09:11
Recombineering-Mediated Genome Editing in Burkholderiales Strains, also closely related Gram-negative bacteria for efficient genome editing. However, some distant bacterial species like Burkholderiales strains require host-specific Redα/Redβ recombinase pair for highly efficient genome editing. A pair of recombinases Redαβ7029 from the Burkholderiales strain DSM 7
用不完
发表于 2025-3-22 06:47:24
High-Efficiency Multi-site Genomic Editing (HEMSE) Made Easy,chnology allows to introduce mutations within bacterial genomes in a very simple and straightforward way. This technology was initially developed for . but was later extended to other organisms of interest, including the environmentally and metabolically versatile .. The technology is based on three
epicondylitis
发表于 2025-3-22 09:16:33
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Hectic
发表于 2025-3-22 13:10:27
Plant Gene Modification by BAC Recombineering,rried by bacterial artificial chromosomes (BACs) in .. Whereas BAC transformation provides a simple means for integration of modified genes into the genomes of animal cells to generate knock-in and knockout lines, successful application of this strategy is hampered by low frequency of homologous rec
BOOST
发表于 2025-3-22 18:56:02
Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling, (DIRex). Dup-In is used for transferring existing mutations between strains, and DIRex for generating almost any type of mutation. Both techniques use intermediate insertions with counter-selectable cassettes, flanked by directly repeated sequences that enable exact and spontaneous excision of the
Barrister
发表于 2025-3-23 01:13:10
Genome Editing in , Using CRISPR/Cas9 Technology,ave recently developed a CRISPR/Cas9-based genome editing system (pCasKP-pSGKP) by coupling a CRISPR/Cas9 system with the lambda Red recombination system as well as a cytidine deaminase-mediated base editing system (pBECKP) in ., enabling rapid, scarless, and efficient genetic manipulation in divers
水獭
发表于 2025-3-23 04:56:41
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歌曲
发表于 2025-3-23 06:13:55
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