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Production and Purification of Antibodies in Chinese Hamster Ovary Cellsal IgG antibodies from Chinese hamster ovary cells. It includes steps for cell maintenance, transient transfection, and antibody purification via protein A affinity chromatography. The methods described are intended for the production of milligram amounts of protein but can be adapted for most small- to mid-scale applications.ALIAS 发表于 2025-3-25 12:39:43
978-1-0716-3668-8The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines广告 发表于 2025-3-25 17:41:38
Recombinant Glycoproteins978-1-0716-3666-4Series ISSN 1064-3745 Series E-ISSN 1940-6029粗糙滥制 发表于 2025-3-25 20:15:31
Steven B. BradfuteIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the expertsAdrenal-Glands 发表于 2025-3-26 00:25:57
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https://doi.org/10.1007/978-1-0716-3666-4Viral-encoded glycoproteins; Mammalian proteins; Carbohydrate groups; Cell-free synthesis; Glycosylation锯齿状 发表于 2025-3-26 09:03:59
Book 2024h understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded glycoproteins and those found in other pathogens, theelastic 发表于 2025-3-26 12:57:18
SARS-CoV-2 S-Protein–Ace2 Binding Analysis Using Surface Plasmon Resonance and flowing monomeric Ace2. This method will allow for the determination of the rate constants for affinity, with additional analysis that is achievable using S-protein capture levels in conjunction with the sensorgram response for relative activity benchmarking.BROW 发表于 2025-3-26 19:17:35
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