Coagulant 发表于 2025-3-21 17:03:18

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Choreography 发表于 2025-3-21 23:12:54

Lawrence M. Mylin,James E. Hopper gradient. The method involves casting a layer of polyacrylamide gel containing a mixture of carrier ampholytes (low-molecular-weight synthetic polyamino—polycarboxylie acids). When an electric field is applied to such a gel, the carrier ampholytes arrange themselves in order of increasing pi from t

污秽 发表于 2025-3-22 04:29:23

Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/824092.jpg

Cupidity 发表于 2025-3-22 05:32:29

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Addictive 发表于 2025-3-22 11:33:21

Strategies for Gene Fusions. The fusion proteins, expressed from such operatively linked genes, often display the combined properties of the parent proteins and are used extensively in microbiology, molecular biology, immunology, and bio-technology (.–.).

一回合 发表于 2025-3-22 15:46:36

Application of the , Promoterproduction levels (.). The . promoter is strong, easily regulated, and well characterized. Transcription of a cloned gene from a . promoter on a plasmid increases about 50-fold upon induction and the gene product can amount to 30% of the total cell protein (.–.).

确定的事 发表于 2025-3-22 19:21:15

Inducible Expression Cassettes in Yeast: ADH2 which are repressed by the presence of glucose. The strength of the ADH2 promoter coupled with the fact that the promoter is tightly regulated make it highly amenable for use in the expression of heterologous genes.

不能和解 发表于 2025-3-23 00:40:04

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infatuation 发表于 2025-3-23 02:01:43

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秘密会议 发表于 2025-3-23 08:11:23

Keshav K. Singh,Jack A. Heinemann involves hydrogen bonding between a few base pairs, the dissociation temperature is very low, near 5°C. Unlike . DNA ligase, T4 DNA ligase will also covalently link two blunt-ended molecules (.C), and this reaction can be used to join ends generated by enzymes that do not give sticky ends.
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查看完整版本: Titlebook: Recombinant Gene Expression Protocols; Rocky S. Tuan Book 1997 Humana Press 1997