ESO
发表于 2025-3-30 09:13:44
Cell Surface Biotinylation of Receptor Tyrosine Kinases to Investigate Intracellular Traffickingace into early endosomes and then travel through intracellular compartments to either recycle back to the membrane or degrade in lysosomes. The biotin-labeled proteins may be detected through affinity purification with streptavidin agarose resins. This chapter describes methods for cell surface biotinylation to assess RTK trafficking steps.
reject
发表于 2025-3-30 14:21:55
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Dislocation
发表于 2025-3-30 20:19:22
Analysis of Epidermal Growth Factor Receptor Dimerization by BS3 Cross-Linkingmers using BS. cross-linking. BS. cross-linking is well suited for this purpose because of its length, water solubility, and membrane impermeability. Although this protocol is written specifically for EGFR, the assay can be extrapolated in order to characterize dimerization of other receptor tyrosine kinases.
去才蔑视
发表于 2025-3-30 22:11:44
Single-Molecule Optical Methods Analyzing Receptor Tyrosine Kinase Activation in Living Cellstal internal reflection fluorescence microscopy (TIRFM), and initial steps of data analysis to identify the time-dependent variation of single-receptor diffusion, which can be widely applied to studying activation of various cell surface receptors.
Temporal-Lobe
发表于 2025-3-31 00:51:29
Human Tumor Xenografts in Mouse as a Model for Evaluating Therapeutic Efficacy of Monoclonal Antibod efficacy of monoclonal antibodies or antibody-drug conjugates specific to human receptor tyrosine kinase using human tumor xenografts in mice as the model. The end point of the study is to determine whether treatment of tumor-bearing mice with a monoclonal antibody or antibody-drug conjugates results in significant delay of tumor growth.
禁止
发表于 2025-3-31 05:35:20
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毕业典礼
发表于 2025-3-31 11:03:15
nvestmentbank „Lehmann Brothers“ 2008, dem Zusammenbruch der internationalen Finanzsysteme und dem Übergreifen der Krise auf die Realwirtschaft wurde eine neue Grundsatzdiskussion über den Umgang mit dem ungezügelten globalen Kapitalismus und die damit verbundene Neuordnung der Märkte entfacht.