渐强
发表于 2025-3-25 04:24:51
Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblottingdecades. Despite the development of quantitative mass spectrometry, reverse-phase protein array, and multiplex technologies, immunoblotting with phospho-specific antibodies is still used in parallel with these technologies and remains a powerful, and reproducible, method for interrogating signaling networks involving RTKs.
macular-edema
发表于 2025-3-25 09:23:47
Quantification of the Effects of Mutations on Receptor Tyrosine Kinase (RTK) Activation in Mammalianotocol for the quantification of the effect of mutations on RTK activation in mammalian cells. The activation measurements are based on Western blotting, and involve direct comparison of receptor phosphorylation under conditions that ensure identical expression of wild-type and mutant receptors.
Contend
发表于 2025-3-25 14:17:54
Downregulation of Receptor Tyrosine Kinases Through Ubiquitination: Analysis by Immunodetectionuate signal transduction. Ubiquitination is a critical step involved in the downregulation of RTK signaling. Here, we describe how to immunodetect the ligand-induced ubiquitination and degradation of TrkA, an RTK, by immunoprecipitation and Western blotting.
GROWL
发表于 2025-3-25 17:42:25
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Highbrow
发表于 2025-3-25 23:56:39
https://doi.org/10.1007/978-1-4939-1789-1Aberrant expression; Negative regulation; Pharmacological targets; Protein expression; RTK activation; RT
转换
发表于 2025-3-26 03:41:05
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commensurate
发表于 2025-3-26 06:08:35
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歪曲道理
发表于 2025-3-26 11:42:13
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包庇
发表于 2025-3-26 16:27:19
Analysis of Changes in Phosphorylation of Receptor Tyrosine Kinases: Antibody Arraysays that are basically used to analyse phosphorylation and dephosphorylation of RTKs. Antibody arrays include well-characterized antibodies for profiling, changes in RTK expression, and comparison between normal, diseased, or treated samples.
植物群
发表于 2025-3-26 19:36:26
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