MOCK
发表于 2025-3-23 12:04:14
Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Bindinghe most part been limited to study of the structure-activity relationships (SAR) of agonists and antagonists at the individual receptors. For a particular receptor, these SAR data were obtained via synthesis of series of chemically modified hgands, followed by the determination of their binding acti
呼吸
发表于 2025-3-23 14:37:00
Approaches to the Stable Transfection of G Protein-Coupled Receptorsnction. The use of stably transfected cell lines enables a receptor to be studied in the absence of other related receptor subtypes. With this technique, molecular determinants of ligand binding can be identified; signal transduction pathways of a particular receptor can be delineated; interactions
杀虫剂
发表于 2025-3-23 21:48:47
Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channelsde variety of proteins (.–.). Transient transfections are especially practical for the expression of recombinant multisubunit complexes because their expression needs the prior knowledge and selection of which of a set of subunits are necessary to form functional heteromultrmers with distinct featur
deficiency
发表于 2025-3-24 01:14:20
The Generation of Receptor-Selective Antibodieshan pharmacological probes. They are often the only probes available that can discern proteins with similar structural and pharmacological properties, such as distinct isoforms or splice-variants of a receptor family. Furthermore, antibodies may be utilized to determine a wide range of a receptor’ c
无聊点好
发表于 2025-3-24 03:33:32
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Harrowing
发表于 2025-3-24 09:34:32
lmmunocytochemical Methods for Investigating Receptor Localizationhe availability of immunochemical probes to simultaneously visualize cellular ultrastructure, such as intracellular organelles, cytoskeleton, proteins involved in signal transduction, and markers of cellular differentiation. The application of immunocytochemical methods, in conjunction with experime
暗讽
发表于 2025-3-24 12:43:53
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exceed
发表于 2025-3-24 17:43:07
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不法行为
发表于 2025-3-24 22:49:41
Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Couplingoteins with altered structures that can be studied for changes in biochemical properties. The most common way to do structure/function studies is by site-directed mutagenesis, in which a particular residue in a protein is selected and systematically altered. Because of the labor-intensive nature of
令人发腻
发表于 2025-3-25 02:57:36
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