退潮
发表于 2025-3-23 11:14:06
Dissecting the Pharmacology of G Protein-Coupled Receptor Signaling Complexes Using Bimolecular Fluque, yet the simplicity of its fluorescent signal holds key advantages for quantification and cellular localization of the resultant complexes..This review introduces general experimental considerations for using the BiFC approach, and describes specific protocols to develop a BiFC assay for GPCR–β-
启发
发表于 2025-3-23 17:30:11
,Measurements of β-Arrestin Recruitment to Activated Seven Transmembrane Receptors Using Enzyme Comp the enzyme, resulting in an enzymatic measure of the protein interaction. This chapter discusses the utility and methods involved in using the PathHunter β-galactosidase complementation system to monitor arrestin recruitment and the advantages of exploiting this pathway in the characterization of 7
汇总
发表于 2025-3-23 21:12:56
Dynamic In Vivo Imaging of Receptors in Small Animals Using Positron Emission Tomography, imaging will significantly reduce the number of animals needed for this type of experiment as well as reducing the effect of inter-animal variation. Experimental protocols in small animal imaging potentially can be very labor intensive. In this chapter, we discuss methods and practical aspects rela
小官
发表于 2025-3-24 01:15:46
Cellular Localization of Receptors Using Antibodies Visualized by Light and Dual Labeling Confocalyet known. The deletion of genes encoding receptors, particularly in mice, has emerged as a powerful tool in understanding the role of a specific receptor in physiological processes. Receptor immunocytochemistry can be used to analyze the resulting phenotype in whole body sections of mice without p
青少年
发表于 2025-3-24 02:47:31
Book 2012Latest editiond hybridization to detect receptor mRNA. Protocols using fluorescent labeled ligands are described to visualise receptors in living cells, their interaction with beta-arrestin to measure ligand-induced internalisation and green fluorescent protein to study trafficking. Non-radioactive, chemilumin
松驰
发表于 2025-3-24 07:26:48
Chidochangu P. Mpamhanga,Joanna L. Sharman,Anthony J. Harmar,NC-IUPHAR
盖他为秘密
发表于 2025-3-24 13:02:00
Janet J. Maguire,Rhoda E. Kuc,Anthony P. Davenport
地名表
发表于 2025-3-24 18:33:31
Daniel L. Bassoni,William J. Raab,Philip L. Achacoso,Chin Yee Loh,Tom S. Wehrman
ARK
发表于 2025-3-24 22:40:38
Peter Johnström,Joseph L. Bird,Anthony P. Davenport
边缘
发表于 2025-3-25 01:46:10
Peter Johnström,Tim D. Fryer,Joseph L. Bird,Hugh K. Richards,Anthony P. Davenport