排他 发表于 2025-3-25 05:37:06

Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of ,,fecting them with in vitro-synthesized dsRNAs. In this chapter we detail the procedure for transient gene silencing in protoplasts using synthetic dsRNAs and provide examples of approaches for subsequent functional analyses.

手榴弹 发表于 2025-3-25 10:40:05

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dysphagia 发表于 2025-3-25 14:23:27

Bisulfite Sequencing for Cytosine-Methylation Analysis in Plants,d lines of transgenic plants (CG>CNG>CHH), while the methylation levels were low in nonsilenced, over-expressing lines. Through grafting, RNA silencing was induced in the non-silenced scions from silenced rootstocks; however, the methylation level of DNA in the scions did not increase.

鄙视读作 发表于 2025-3-25 17:54:38

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搜寻 发表于 2025-3-25 21:31:40

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Aesthete 发表于 2025-3-26 03:16:38

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Mitigate 发表于 2025-3-26 04:47:02

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evince 发表于 2025-3-26 08:35:34

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流逝 发表于 2025-3-26 14:57:15

Using Nuclear Run-On Transcription Assays in RNAi Studies,led transcripts that then can be hybridized to filter-bound, cold, excess single-stranded DNA probes representing genes of interest. The protocol presented here streamlines, adapts, and optimizes nuclear run-on transcription assays for use in RNAi studies.

上流社会 发表于 2025-3-26 17:03:14

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查看完整版本: Titlebook: RNAi and Plant Gene Function Analysis; Methods and Protocol Hiroaki Kodama,Atsushi Komamine Book 2011 Springer Science+Business Media, LLC