值得
发表于 2025-3-26 22:05:59
In Situ Hybridization Detection of microRNAs,scribe a protocol for miRNA in situ hybridization on mouse tissue cryosections using locked nucleic acid (LNA) probes. LNA probes demonstrate a much higher affinity to their complimentary RNAs compared to conventional RNA and DNA oligo probes, which allow detection of miRNAs in tissue sections with excellent specificity.
睨视
发表于 2025-3-27 01:19:31
1064-3745 rapeutic proteins.Features the translation of RNA-based ther.Central to the synthesis of proteins, the performance of catalysis, and many other physiological processes, the aberrant expression of which can be linked to human diseases including cancers, RNA has proven to be key target for therapeutic
magnanimity
发表于 2025-3-27 06:54:12
Advances in RNA Sensing by the Immune System: Separation of siRNA Unwanted Effects from RNA Interfereduced the number of unwanted off-target genes without interfering with siRNA silencing potency of the anticipated target gene. This chapter describes how to separate gene silencing from immunostimulation. Also, it discusses the impact of these findings on the design of effective cancer vaccines.
Gastric
发表于 2025-3-27 10:21:56
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分开
发表于 2025-3-27 14:47:40
Quantitative Analysis of RNA Modifications,. Here, we present a detailed procedure that allows the detection and quantification of RNA base modifications. This procedure involves a number of techniques, including oligonucleotide-affinity selection, site-specific cleavage and radiolabeling, nuclease digestion, and thin layer chromatography.
背信
发表于 2025-3-27 20:46:21
Systemic Delivery of Synthetic siRNAs,llenge have been described, including cationic lipids, high-pressure injection, viral vectors, and chemical modifications of the siRNAs. This chapter describes cationic lipid delivery of siRNAs to adult mice.
半球
发表于 2025-3-28 00:46:20
Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migratio cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.
侵略
发表于 2025-3-28 05:45:20
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讲个故事逗他
发表于 2025-3-28 09:58:09
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软膏
发表于 2025-3-28 11:18:55
RNA Modifications: A Mechanism that Modulates Gene Expression,l modifications, including 2ʹ-O-methylation and pseudouridylation, which are functionally important. In this chapter, we highlight the functional aspects of the modifications of spliceosomal snRNA and rRNA and provide a framework for understanding how posttranscriptional modifications are capable of influencing gene expression.