BUOY 发表于 2025-3-26 23:02:41
Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820197.jpg察觉 发表于 2025-3-27 05:03:37
https://doi.org/10.1007/978-1-0716-2687-0X-ray Crystallography; XFEL; riboswitches; NMR; RNA conformational trajectory表皮 发表于 2025-3-27 07:11:29
Characterizing Fluorescence Properties of Turn-on RNA Aptamers,s should be characterized prior to cellular imaging experiments. Here, we describe the process of determining the fluorophore affinity, fluorescence enhancement, and fluorescence lifetime(s) of the Mango-III fluorescence turn-on aptamer. Parameters determined through these protocols will aid in establishing conditions for live-cell imaging.成绩上升 发表于 2025-3-27 11:33:35
Isothermal Titration Calorimetry Analysis of a Cooperative Riboswitch Using an Interdependent-Sites alteration of the underlying chemical structures. In this protocol, we describe procedures for performing, analyzing, and interpreting ITC data obtained from a cooperative riboswitch–ligand interaction.Meager 发表于 2025-3-27 13:42:32
http://reply.papertrans.cn/83/8202/820197/820197_35.pngHamper 发表于 2025-3-27 20:02:13
Probing Transient Riboswitch Structures via Single Molecule Accessibility Analysis, monitored by Total Internal Reflection Fluorescence Microscopy (TIRFM) and quantitatively analyzed, via spike train and burst detection, to elucidate the rate constants of isomerization, revealing mechanistic insights into riboswitching.BRAWL 发表于 2025-3-27 23:10:50
http://reply.papertrans.cn/83/8202/820197/820197_37.pngNarrative 发表于 2025-3-28 05:16:07
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http://reply.papertrans.cn/83/8202/820197/820197_39.png绝食 发表于 2025-3-28 11:03:25
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