Wilson 发表于 2025-3-21 16:16:14
书目名称RNA影响因子(影响力)<br> http://impactfactor.cn/if/?ISSN=BK0820144<br><br> <br><br>书目名称RNA影响因子(影响力)学科排名<br> http://impactfactor.cn/ifr/?ISSN=BK0820144<br><br> <br><br>书目名称RNA网络公开度<br> http://impactfactor.cn/at/?ISSN=BK0820144<br><br> <br><br>书目名称RNA网络公开度学科排名<br> http://impactfactor.cn/atr/?ISSN=BK0820144<br><br> <br><br>书目名称RNA被引频次<br> http://impactfactor.cn/tc/?ISSN=BK0820144<br><br> <br><br>书目名称RNA被引频次学科排名<br> http://impactfactor.cn/tcr/?ISSN=BK0820144<br><br> <br><br>书目名称RNA年度引用<br> http://impactfactor.cn/ii/?ISSN=BK0820144<br><br> <br><br>书目名称RNA年度引用学科排名<br> http://impactfactor.cn/iir/?ISSN=BK0820144<br><br> <br><br>书目名称RNA读者反馈<br> http://impactfactor.cn/5y/?ISSN=BK0820144<br><br> <br><br>书目名称RNA读者反馈学科排名<br> http://impactfactor.cn/5yr/?ISSN=BK0820144<br><br> <br><br>Initial 发表于 2025-3-21 21:29:01
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Working with RNAk rules have to be implemented to maintain the integrity of the RNA. Alkaline pH, high temperatures, and heavy metal ions should be avoided when possible and ribonucleases kept in check. The chapter outlines the specific precautions recommended for work with RNA and describes some of the modificatioantidepressant 发表于 2025-3-22 07:57:22
Synthesis of RNA by In Vitro Transcriptionto those of several kilobases in μg to mg quantities. It is based on the engineering of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymerase. In vitro transcripts遗传 发表于 2025-3-22 11:08:29
Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) Capturepoly(A). RNA directly from guanidinium thiocyanate (GuSCN)-containing cell or tissue extract by combining the design of biotinylated LNA oligo(T) capture probes with subsequent immobilization of the captured poly(A). RNA onto streptavidin-coated magnetic particles. In contrast to DNA oligo-dT and poATRIA 发表于 2025-3-22 15:39:14
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Rapid Amplification of cDNA Ends (RACE)ly known. Starting with an mRNA mixture, gene-specific primers generated from the known regions of the gene and non-specific anchors, full-length sequences can be identified in as little as 3 days. RACE can also be used to identify alternative transcripts of a gene when the partial or complete seque整顿 发表于 2025-3-23 07:45:34
Fractionation of mRNA Based on the Length of the Poly(A) Taile mRNAs according to the length of the poly(A) tail. Poly(A) fractionation can be used to detect small changes in poly(A) tail length or to prepare samples for microarray analysis. RNA or crude lysate is mixed with biotinylated oligo(dT), which is then bound to paramagnetic streptavidin beads. Oligo