BARB
发表于 2025-3-25 06:29:39
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cardiovascular
发表于 2025-3-25 11:30:11
Synthesis of cDNAFor detection or quantitation of mRNA by polymerase chain reaction, it is necessary to transcribe mRNA into copy-DNA (cDNA) by a reverse transcriptase (RT) reaction. The quality of a cDNA depends on the integrity of the messenger RNA and the fidelity of the transcription.
不可比拟
发表于 2025-3-25 12:56:54
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clarify
发表于 2025-3-25 18:24:42
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AGGER
发表于 2025-3-25 22:02:09
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博识
发表于 2025-3-26 03:52:07
Quantitation of Absolute Numbers of mRNA Copies in a cDNA Sample by Competitive PCRIn this chapter a competitive PCR assay using an internal DNA standard is described. Competitive PCR is a quantitative adaptation of the PCR method in which a known number of copies of an exogenous synthesized and added DNA (or RNA) is amplified together with the sample in the same PCR tube (see chapter 1.1).
旧式步枪
发表于 2025-3-26 07:49:36
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Muffle
发表于 2025-3-26 08:30:21
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合唱队
发表于 2025-3-26 15:51:23
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archetype
发表于 2025-3-26 20:52:22
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