inroad 发表于 2025-3-21 17:10:32
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Exact Theory of Optical Tweezers and Its Application to Absolute Calibrationyleigh regime to the ray optics one, for different polarizations and trapping heights, including all commonly employed parameter domains. Protocols for implementing absolute calibration are given, explaining how to measure all required experimental parameters, and including a link to an applet for stiffness calculations.resistant 发表于 2025-3-22 02:00:27
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Introduction to Optical Tweezerstific achievements, and demonstrate why optical traps have become a powerful tool in the biological sciences. We introduce a typical optical setup, describe the basic theoretical concepts of how trapping forces arise, and present the quantitative position and force measurement techniques that are most widely used today.大火 发表于 2025-3-22 10:36:02
Optical Torque Wrench Design and Calibrationrientation, described by a periodic potential. As a consequence of the experimental control of the tilt of the periodic potential, the dynamical excitability of the system can be observed. Angular optical tweezers remain less widespread than their linear counterpart. We hope this technical guide can foster their development and new applications.Fortuitous 发表于 2025-3-22 16:31:46
Probing Single Helicase Dynamics on Long Nucleic Acids Through Fluorescence-Force Measurementof fluorescence-force spectroscopy instrumentation, calibration, and activity assays in detail for observing the biochemical activities of helicases in real time and revealing how mechanical forces are involved in protein–nucleic acid interaction. These single-molecule approaches are generally applicable to many other protein–nucleic acid systems.粘连 发表于 2025-3-22 18:28:42
Book 2017sts, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation .in vitro. and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed pro价值在贬值 发表于 2025-3-23 01:10:08
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Implementation and Tuning of an Optical Tweezers Force-Clamp Feedback Systemliance of the involved molecules. To investigate a particular mechanical process, understanding the basics and limitations of the feedback system will be helpful for choosing the proper feedback hardware, for optimizing the system parameters, and for the design of the experiment.恸哭 发表于 2025-3-23 06:10:10
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