搭话 发表于 2025-3-21 19:21:14
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Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acls, stored indefinitely at −20°C, have full lactate dehydrogenase activity (.) and DNA polymerase activity (.). The dried cells stored for a few days at room temperature also have apparently undegraded nucleic acids (.).Aprope 发表于 2025-3-22 02:56:52
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Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions,lecules is left intact during electrophoresis. The first method describes electrophoresis in a 2% (w/v) agarose gel in a dilute, neutral phosphate buffer. The second deals with electrophoresis in a linear gradient of polyacrylamide based on the buffer system of Loening (.).BACLE 发表于 2025-3-22 11:05:18
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Messenger RNA Fractionation on Neutral Sucrose Gradients,duction of poly(A). mRNA by affinity chromatography (..) the RNA can be fractionated once or twice on sucrose gradients to produce a subpopulation of mRNA enriched for a particular species. The RNA fractions from the gradient can be assayed by in vitro translation and subsequent immunoprecipitation or bioassay of the products (..–.).Mhc-Molecule 发表于 2025-3-22 18:02:43
Nucleic Acids978-1-59259-489-4Series ISSN 1064-3745 Series E-ISSN 1940-6029憎恶 发表于 2025-3-22 23:55:17
,Preparation of “RNase-Free” DNase by Alkylation,Characterization of RNA molecules by electrophoresis or hybridization frequently requires nucleic acid concentrations over 1 mg/mL. High molecular weight DNA in a mixture of nucleic acids limits the solubility and interferes with electrophoresis. DNase treatment makes the mixture more soluble, even if DNA degradation is only partial.无效 发表于 2025-3-23 02:51:55
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Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/668784.jpg