公式 发表于 2025-3-23 13:05:59
Generation of Stable Amber Suppression Cell Linesediated transposition. A general protocol for the generation of stable amber suppression cell lines is described here. Using the modular plasmid system, homogenous and highly efficient amber suppression in a wide range of cell lines can be achieved.弄脏 发表于 2025-3-23 17:22:43
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http://reply.papertrans.cn/67/6672/667175/667175_13.pngosteoclasts 发表于 2025-3-24 00:55:53
978-1-4939-8525-8Springer Science+Business Media, LLC 2018Climate 发表于 2025-3-24 06:25:58
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Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/667175.jpg孤独无助 发表于 2025-3-24 18:46:03
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Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugatemylglycine-generating enzyme (FGE) is one enzyme that can be exploited in this fashion. FGE binds its consensus sequence (CXPXR, known as the “aldehyde-tag”) and converts the cysteine to a formylglycine (fGly). fGly-containing proteins contain a bioorthogonal aldehyde on their surface that can be mo