母猪
发表于 2025-3-25 07:14:17
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考得
发表于 2025-3-25 08:44:28
Computer Applications to Molecular Biology,ed, and the most recent issue of the . (release 10, 1987) contains almost 10 million base pairs in almost 9000 entries compared with the first release from 1982 containing half a million base pairs in 500 entries. The requirement for computers is obvious not only from the total amount of DNA sequenc
Chronological
发表于 2025-3-25 13:04:14
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Perigee
发表于 2025-3-25 18:00:53
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加强防卫
发表于 2025-3-25 23:01:43
Manual Oligonucleotide Synthesis Using the Phosphoramidite Method,exocyclic amino groups on cytosine and the purines are protected as the alkali-labile benzoyl or .-butyryl amides. The 3′-OH group of the first nucleoside is attached to the solid support by a spacer chain, and the synthesis procedes by coupling the 5′-OH group of the growing chain to the 3′-phospho
联邦
发表于 2025-3-26 02:45:28
Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis,uction of novel linkers, site-directed mutagenesis, and many other techniques. The material obtained from a particular synthesis contains traces of premature termination products and other unwanted byproducts of the organic reactions. For most uses in genetic engineering, the material must be purifi
libertine
发表于 2025-3-26 06:44:59
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小卒
发表于 2025-3-26 11:53:11
,Construction of Mammalian Genomic Libraries Using λ Replacement Vectors,ideal state can be approached by cutting the DNA randomly and cloning large pieces of this DNA into a suitable vector (.). The DNA can be cleaved either by mechanical shearing, in which case the DNA is fragmented in a truely random fashion, but with the introduction of problems associated with blunt
责难
发表于 2025-3-26 14:57:22
Cosmid Library Construction,ely from bacteriophage λ, where nonessential intergenic regions were identified. It had been noted that only λ DNA 37–52 kb in length (or 78–102% of the wild-type length) were efficiently packaged and passaged in vivo. Thus vectors from which the intergenic region had been removed could be positivel
伦理学
发表于 2025-3-26 17:15:44
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