预兆好
发表于 2025-3-23 11:25:45
Preparation of Fragaceatoxin C (FraC) Nanopores via two rounds of Ni-NTA affinity chromatography before and after oligomerization on sphingomyelin-containing liposomes. Starting from a plasmid vector containing the FraC gene, our method allows the production of purified nanopores within a week. Afterward, the FraC nanopores can be stored at +4 °C for several months, or frozen.
ANIM
发表于 2025-3-23 14:09:14
Nanopore Enzymology to Study Protein Kinases and Their Inhibition by Small Moleculesnd production of suitably engineered protein nanopores and their use for the determination of key mechanistic parameters of kinases. We also provide procedures for the determination of inhibition constants of protein kinase inhibitors.
ALIAS
发表于 2025-3-23 21:19:35
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水槽
发表于 2025-3-23 23:33:22
Oligonucleotide-Directed Protein Threading Through a Rigid Nanoporepore inserted in an electrically insulating membrane. The translocation of the protein molecule through the pore causes a modulation of the ionic current. Analysis of the ionic current reveals the biophysics of co-translocational unfolding and may be used to infer the amino acid sequence and posttranslational modifications of the molecule.
较早
发表于 2025-3-24 03:48:50
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hieroglyphic
发表于 2025-3-24 07:01:19
Design and Assembly of Membrane-Spanning DNA NanoporesVersatile lipid membrane-inserting nanopores have been made by functionalizing DNA nanostructures with hydrophobic tags. Here, we outline design and considerations to obtain DNA nanopores with the desired dimensions and conductance properties. We further provide guidance on their reconstitution into lipid membranes.
Postulate
发表于 2025-3-24 12:33:21
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charisma
发表于 2025-3-24 15:57:48
Nanopore Technology978-1-0716-0806-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
敲竹杠
发表于 2025-3-24 20:54:05
Methods in Molecular Biologyhttp://image.papertrans.cn/n/image/660911.jpg
amnesia
发表于 2025-3-24 23:09:57
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