孵化
发表于 2025-3-21 17:41:48
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Blood-Clot
发表于 2025-3-21 22:10:57
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evince
发表于 2025-3-22 00:28:48
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停止偿付
发表于 2025-3-22 04:34:12
Antisense RNA Elements for Downregulating Expression,yl-carrier-protein] transacylase as an example to describe the asRNA design with reliable and controllable interference efficiency. Real-time PCR and fluorescence assay methods are introduced to detect the interference efficiency at RNA level and protein level, respectively.
爆米花
发表于 2025-3-22 09:28:41
Chemically Inducible Chromosomal Evolution (CIChE) for Multicopy Metabolic Pathway Engineering, recombination. A strong selective pressure for gene duplication can be applied by increasing antibiotic concentration, and in a week’s time one can create a set of strains with a wide range of cassette copy numbers (upward of 20×), which can be made stable by deletion of .. Herein, we describe a generalized workflow for this methodology.
anthropologist
发表于 2025-3-22 16:15:53
Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant , detailed including methods to test promoter strength using the green fluorescent protein reporter. Furthermore, a method for markerless transformation of chromosomal DNA is presented. Sufficient details are provided to enable application by the novice investigator.
宪法没有
发表于 2025-3-22 19:33:41
1064-3745 ation advice from the expertsThis volume covers a wide array of topics that will aid researchers in the task of engineering complex biological systems. This book is divided into three parts: Part One discusses the discovery and identification of relevant biosynthetic pathways for engineering; Part T
CLASP
发表于 2025-3-22 23:46:10
Assembly of Complex Pathways Using Type IIs Restriction Enzymes,red for the construction strategy. Basic parts that are not yet available need to be made. Multigene constructs are then assembled using a series of one-pot assembly steps with the set of identified parts and vectors.
Defraud
发表于 2025-3-23 03:06:48
Checks and Balances with Use of the Keio Collection for Phenotype Testing,es that can be employed to ensure robust phenotype assessment of mutations in the library. These include procedures for thorough confirmation of gene deletions by PCR, phage transduction of mutated loci to new host strains, and strategies for genetic complementation.
庇护
发表于 2025-3-23 05:53:13
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