tornado 发表于 2025-3-21 19:13:35
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Analytical and Preparative Polyacrylamide Gel Electrophoresis,se species first in two-component systems, then in ever more complex systems and finally to reconstitute life as the sum of its component chemical reactions. The first task, i.e., the isolation of nature’s building-block molecules, remains a formidable task. This chapter is concerned with the instru大包裹 发表于 2025-3-22 02:34:09
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Estimation of Molecular Weight by Gel Filtration and Gel Electrophoresis,tein molecular weights. However, the precision and accuracy of these methods have never been rigorously evaluated by formal statistical methods. In contrast, a detailed objective statistical analysis has been applied to PAGE in the absence of SDS (Rodbard and Chrambach, 1970, 1974). This statisticalThyroid-Gland 发表于 2025-3-22 09:18:20
Zone Electrophoresis, Isoelectric Focusing, and Displacement Electrophoresis (Isotachophoresis) in of some more-or-less rigid supporting medium, the presence of which can, however, have several undesirable consequences: (1) The solute of interest is adsorbed, leading to low recoveries and (partial) loss of biological activity. (2) Cells and many other particles are too large to penetrate electropGrating 发表于 2025-3-22 13:42:38
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Hydrophobic Adsorption Chromatography of Proteins,s a parameter for nonspecific adsorption chromatography of proteins. One of the reasons for the delay of investigations of possible hydrophobic effects probably was the assumption that hydrophobic groups of proteins generally are situated in the interior of the native protein molecule and thus are i偶然 发表于 2025-3-22 22:22:27
Sievorptive Chromatography,s ability provides a powerful incentive to continually improve the procedures for the isolation of macromolecules. The scope of biological separations is overwhelmingly diverse. Even when the discussion is restricted to proteins, the problems range from the separation of macromolecules that strongly迅速成长 发表于 2025-3-23 04:15:59
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