penance
发表于 2025-3-25 04:58:25
http://reply.papertrans.cn/64/6325/632414/632414_21.png
Anemia
发表于 2025-3-25 10:14:29
http://reply.papertrans.cn/64/6325/632414/632414_22.png
Infantry
发表于 2025-3-25 15:35:52
http://reply.papertrans.cn/64/6325/632414/632414_23.png
提名的名单
发表于 2025-3-25 17:37:08
Analysis of Phenylthiohydantoin Amino Acids by HPLC. It is the only technique commonly used for PTH analysis that can resolve all of the PTH amino acids in a single chromatographic run. Because it provides quantitative data at the pmol level, HPLC is also the only available method suitable for microsequencing that uses the new generation of automated Edman sequencers (.,.).
Interregnum
发表于 2025-3-25 22:19:44
http://reply.papertrans.cn/64/6325/632414/632414_25.png
initiate
发表于 2025-3-26 01:47:02
Manual Edman Sequencing Systementific discipline, existing as it does merely to supply probes to the molecular geneticists and occasionally to check their DNA reading frames, and one has a method in deep psychological crisis. Fortunately, manual sequencing can provide any of the structural data that requires or advantageously utilizes protein chemistry.
宽大
发表于 2025-3-26 06:27:05
Gas-Phase Protein/Peptide Sequencer). The resulting amino acid derivative-an anilinothiazolinone (ATZ)- is converted to the more stable phenylthiohydantoin (PTH) amino acid, which is analyzed by high performance liquid chromatography (HPLC). The remaining peptide is subjected to further degradation cycles to generate its amino acid sequence.
Genome
发表于 2025-3-26 09:47:12
Design of a Multipurpose Sequencer-called liquid-phase technique, in which the protein is degraded as thin film in a spinning cup, has been widely used and successfully applied to the sequence determination of proteins and polypeptide fragments.
nitroglycerin
发表于 2025-3-26 15:55:24
http://reply.papertrans.cn/64/6325/632414/632414_29.png
先驱
发表于 2025-3-26 18:00:40
COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methodsrminal modifications, especially for many regulatory pep-tides (.,.). Thus, whenever Edman degradation is used as the prinicpal method of complete amino acid sequence determination, it must be supplemented by COOH-terminal analysis.