Callus
发表于 2025-3-23 11:06:25
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侵害
发表于 2025-3-23 15:44:36
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教唆
发表于 2025-3-23 20:35:10
Culture Methods for the Selection and Isolation of Stable Antibody-Producing Murine Hybridomas,selection and expansion of fusion cultures. The later procedure provides for reliable estimation of the stability of hybrid clones and ensures the identification of hybridomas that continue to secrete antibody.
overreach
发表于 2025-3-24 01:22:16
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instructive
发表于 2025-3-24 04:38:48
Statistical Assessment of Hybridoma Monoclonality After Subcloning by the Limiting Dilution Techniqlishing the monoclonality of their hybridomas. Although the application of the Poisson distribution for statistically analyzing a single subcloning has been described in great detail (2,3), in hybridoma studies it is customary to subclone at least twice and the statistics for repetitive subclonings
参考书目
发表于 2025-3-24 08:28:06
In Vitro Immunization for the Generation of Hybridomas Using Serum-Free Medium,operties. One approach to the improvement of hybridoma formation techniques is to attempt to define the components and reaction conditions that result in maximal formation of antibody-secreting hybridomas. Until recently, all aspects of in vitro immunization and hybridoma formation have involved an
Dorsal
发表于 2025-3-24 13:07:41
Human Hybridomas,es secreting human antibody. However, the loss of human chromosomes from mouse x human hybridomas is not random. It is known, for example, that human chromosomes 14 and 22, which bear the Ig heavy chain and λ light chain locus, respectively, are preferentially retained, whereas chromosome 2, bearing
Confound
发表于 2025-3-24 18:35:23
Antibody-Secreting Human-Human Hybridomas,lone in semisolid or liquid medium, and they frequently lose their Ig secretion after a few months in culture (3,6). Considerable efforts have therefore been invested to develop improved malignant fusion partners.
macrophage
发表于 2025-3-24 20:01:50
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Conflagration
发表于 2025-3-25 01:38:38
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