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Detectors for Fluorescence Microscopytical components in fluorescence microscopy is the imaging device because it determines whether the fluorescence may be detected, the relevant structures resolved, or the dynamics of a process visualized (Aikens et al., 1988; Becker, 1996; Bookman, 1990; Bright and Taylor, 1986; Herman, 1998; PeriasCARE 发表于 2025-3-22 05:28:31
Basics of a Light Microscopy Imaging System and Its Application in Biologyernike, 1958), differential interference contrast (Allen et al., 1969), epifluorescence (Ploem, 1967), laser scanning confocal (Minsky, 1957), and now multiphoton confocal microscopy (Denk et al., 1990). As the imaging technology evolves (Inoué and Spring, 1997), the biologist works to prepare fixedConstituent 发表于 2025-3-22 11:30:11
Laser Scanning Confocal Microscopy Applied to Living Cells and Tissuesitude and time course of individual cellular changes. Responses within single cells may also show spatial heterogeneity. For these reasons, three-dimensionally resolved approaches are needed to study individual cells as they respond to imposed stimuli and stresses. Increasingly, confocal microscopyBOON 发表于 2025-3-22 16:09:39
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Basic Principles of Multiphoton Excitation Microscopy GöppertMayer in 1931. Generating three-dimensionally resolved microscopic images based on nonlinear optical excitation was postulated in the 1970s (Gannaway and Sheppard, 1978; Wilson and Sheppard, 1984). The definitive experiment was done by Denk, Webb, and co-workers (1990), who accomplished two-泛滥 发表于 2025-3-23 06:34:26
Building a Two-Photon Microscope Using a Laser Scanning Confocal Architecture al., 1990), two-photon excitation (TPE) fluorescence microscopy can be considered a comparatively young technique in far-field fluorescence optical microscopy. This technique has advantages over both widefield and confocal laser scanning microscopy (Wilson and Sheppard, 1984; Wilson, 1990; Pawley,