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Measuring the Nutrient Metabolism of Adherent Cells in Culture,tured adherent mammalian cells to collect both polar and nonpolar intracellular metabolites. This chapter also describes experimental design considerations for performing stable isotope labeling experiments, and the use of chemical derivatization to increase the number of compounds that can be detected using one chromatography method.Flounder 发表于 2025-3-22 18:26:16
13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells,orage-independent growth, spheroid formation, and extracellular matrix production by (cancer) cells. Here, we describe how to perform metabolomics and .C tracer analysis in 3D cultures of cancer cells.冥界三河 发表于 2025-3-22 23:28:11
Book 2019ucible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. . Authoritative and cutting-edge, .Metabolic Signaling: Methods and Protocols. aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level..不知疲倦 发表于 2025-3-23 03:03:59
Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells Using Radioactive Tracers,e, but also for the identification of anti-angiogenic metabolic targets. This chapter describes in detail the radioactive tracer substrate assays that have been used for the determination of EC metabolic flux in vitro.集聚成团 发表于 2025-3-23 08:17:38
Development of Patient-Derived Tumor Xenograft Models,ed mice, allowing for the progressive growth and amplification of cancer tissue for in vivo testing. Here, we describe the detailed methods we developed to establish PDX from both surgically removed endometrial cancer fragments (endometrial cancer) and fine-needle aspiration biopsies (pancreatic cancer).