尾巴 发表于 2025-3-28 17:05:59
http://reply.papertrans.cn/64/6305/630462/630462_41.png栏杆 发表于 2025-3-28 21:42:28
http://reply.papertrans.cn/64/6305/630462/630462_42.pngmosque 发表于 2025-3-29 01:06:21
Epigenomic Profiling of B Cell Subsets by CUT&Tag mechanisms, such as the post-translational modifications to histones, present several technical challenges that prevent a complete understanding of gene regulation. Here, we provide a detailed protocol of the Cleavage Under Targets and Tagmentation (CUT&Tag) chromatin profiling technique for identiGROWL 发表于 2025-3-29 04:02:54
http://reply.papertrans.cn/64/6305/630462/630462_44.pngExclaim 发表于 2025-3-29 09:56:39
Isolation of Rare Antigen-Specific Memory B Cells via Antigen Tetramerseeper understanding of the mechanisms underlying this phenomenon can aide in research aimed to improve vaccines and therapies. Memory B cells (MBCs) are a fundamental component of immunological memory but can exist in rare populations that prove challenging to study. By combining fluorescent antigenCUB 发表于 2025-3-29 13:29:23
http://reply.papertrans.cn/64/6305/630462/630462_46.pngGLUE 发表于 2025-3-29 19:19:02
ELISpots for Detecting Antigen-Specific Memory B Cells in Infection or Autoimmunitycells cultured in ELISpot plates (96- or 8-well format) bind to a specific antigen bound to a PVDF membrane at the bottom of the well. A detection antibody followed by an enzymatic reaction is used to identify secreted protein bound to the membrane coated antigen. This reaction results in distinct “鞭子 发表于 2025-3-29 23:00:26
http://reply.papertrans.cn/64/6305/630462/630462_48.pngSPECT 发表于 2025-3-30 00:11:18
http://reply.papertrans.cn/64/6305/630462/630462_49.png其他 发表于 2025-3-30 08:07:27
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