不来 发表于 2025-3-25 06:42:27
Labeling of Membrane Complexes for Electron Microscopy,tic nature of their subunits and their modular organization results in difficult genetic manipulation and instability upon purification. Here, we present different labeling approaches that have been demonstrated successful in the structural characterization of large membrane complexes.密码 发表于 2025-3-25 09:43:16
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Mass Spectrometry of Mitochondrial Membrane Protein Complexes, model for the study of oxidative phosphorylation; mutants are easy to produce and are still viable due to their ability to grow using the fermentation pathway. Here, we present a process for analyzing mitochondrial respiratory complexes using native electrophoresis (BN-PAGE) coupled to LC-MS/MS. BN细胞 发表于 2025-3-26 07:05:46
Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in membrane proteins, independent of size or the presence of detergent or lipids. Slow, medium, and fast exchanging domains are distinguished, which reveal a different accessibility to the solvent. Whereas amide hydrogens undergo rapid exchange with solvent in an open structure, hydrogens experience m温和女人 发表于 2025-3-26 10:30:52
http://reply.papertrans.cn/64/6304/630382/630382_28.pngHemodialysis 发表于 2025-3-26 14:17:03
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The CRACAM Robot: Two-Dimensional Crystallization of Membrane Protein,de structural experimental information at atomic scale for membrane proteins forming two-dimensional (2D) crystals. There are two different methods to produce 2D crystals of membrane proteins. (1) either directly in the bulk of the solution (2) or under a lipid monolayer at the air-water interface.