tenosynovitis
发表于 2025-3-23 10:03:03
Reconstitution of Membrane Proteins into Nanodiscs for Single-Particle Electron Microscopy,ess to structural methodologies developed for soluble proteins. Here, we present the reconstitution of OprM membrane protein into nanodisc associated with a step of size-exclusion chromatography, an approach applicable to prepare IMPs for subsequent visualization by single-particle electron microscopy.
Basilar-Artery
发表于 2025-3-23 16:06:54
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monochromatic
发表于 2025-3-23 20:49:41
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先兆
发表于 2025-3-23 23:14:15
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Tdd526
发表于 2025-3-24 06:10:17
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使虚弱
发表于 2025-3-24 06:48:43
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来就得意
发表于 2025-3-24 11:11:56
Direct Extraction and Purification of Recombinant Membrane Proteins from , Protoplasts,ne proteins (MPs), leading to the crystallization and structure determination for a variety of them. The actual overexpression of functional MPs achieved with this system is, however, often accompanied by the formation of a variable but significant proportion of misfolded and/or aggregated proteins
去掉
发表于 2025-3-24 18:53:23
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forebear
发表于 2025-3-24 20:15:33
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dandruff
发表于 2025-3-24 23:35:40
Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins,provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter, we discuss the approach for labeling methyl groups in .-based protein expression, as exemplified with the mitochondrial carrier GGC.