Interpolate 发表于 2025-3-21 19:55:01
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Book 2017Latest editionl methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies缩短 发表于 2025-3-22 03:29:02
Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in ,,nation and chromosome segregation. Here we describe genetic methods for analysis progression of . through meiosis and sporulation with an emphasis on strategies for the genetic analysis of regulators of meiosis-specific genes.preservative 发表于 2025-3-22 08:02:23
Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy,ytes in detail as they undergo maturation. Our method is based on tracking the “center of brightness” of fluorescently labeled chromosomes. Here we describe how to set up our software and run experiments on a Leica TCS SP8 confocal microscope, but the method would be transferable to other microscopes with computer-aided microscopy.过多 发表于 2025-3-22 12:20:44
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Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast,tions of DNA breaks to the base pair. In this chapter we detail the steps involved in Spo11-oligo mapping in the SK1 strain of budding yeast ., from harvesting cells of highly synchronous meiotic cultures, through preparation of sequencing libraries, to the mapping pipeline used for processing the data.湿润 发表于 2025-3-23 00:08:57
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Fluorescent Protein as a Tool for Investigating Meiotic Recombination in ,,nalysis of recombination outcomes by allowing visualization of segregation patterns in a large number of octads from crosses heterozygous for .. Using this system the effect of a knockout on gene conversion and/or on crossing over between the fluorescent marker and the centromere can be measured.民间传说 发表于 2025-3-23 05:45:10
In Vivo Imaging of Budding Yeast Meiosis,is method allows tracking of individual cells over extended periods of time through every stage of the meiotic transformation while minimizing phototoxicity and sustaining conditions that support meiotic growth.