明显
发表于 2025-3-21 16:04:37
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性冷淡
发表于 2025-3-21 23:58:06
Multilocus Sequence Typing (MLST) of ,r methods as it can reconstruct ancestral and evolutionary linkage between . isolates. MLST detects all genetic variations within the amplified housekeeping gene that accumulate slowly. This chapter describes how to undertake MLST.
明智的人
发表于 2025-3-22 01:10:39
Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of ,ated to specific double-stranded adaptors. The ligated fragments are then amplified by PCR using fluorescently labelled primers. Fluorescent amplified fragments are separated by size on an automated sequencer with a size standard. fAFLP is a rapid, highly reproducible technique which can be used to discriminate and subtype . strains.
Ardent
发表于 2025-3-22 07:05:39
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fulmination
发表于 2025-3-22 09:52:22
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口诀法
发表于 2025-3-22 13:14:30
Generation of Nonpolar Deletion Mutants in , Using the “SOEing” Method. For mutagenesis in ., we describe two different plasmid-based approaches, which facilitate the introduction of this spliced amplicon in place of the corresponding segment of chromosomal DNA: the pKSV7 system and the pORI280/pVE6007 system.
LIKEN
发表于 2025-3-22 17:45:30
Sampling the Processing Environment for ,to sample, the best time for sampling and the frequency of sampling are also given. Such details help hygienists/microbiologists to be successful in their attempts to isolate strains of ., even if such bacteria are well attached to surfaces or located in niches that are difficult to reach.
ANA
发表于 2025-3-22 21:33:46
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含铁
发表于 2025-3-23 04:15:53
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ROOF
发表于 2025-3-23 08:17:41
Extraction and Analysis of Plasmid DNA from ,ient for analytical purposes but may be upscaled for further downstream analysis. Electrophoretic separation of the resultant lysate allows conclusions to be made on the presence, number, copy number, and size of the plasmids in the analyzed bacterial strains.