微不足道 发表于 2025-3-30 11:12:29
http://reply.papertrans.cn/59/5851/585006/585006_51.pngJogging 发表于 2025-3-30 13:01:59
Purification of LPS from ,to extract and/or purify the leptospiral lipopolysaccharides: the rapid proteinase K method and the classical hot water/phenol extraction. The first method allows to get a quick overview of the LPS O antigen structure, whereas the second method is fitted to study the immunological properties of thedissent 发表于 2025-3-30 17:57:42
http://reply.papertrans.cn/59/5851/585006/585006_53.pngInfiltrate 发表于 2025-3-30 21:02:28
http://reply.papertrans.cn/59/5851/585006/585006_54.png疏忽 发表于 2025-3-31 02:35:39
http://reply.papertrans.cn/59/5851/585006/585006_55.png薄膜 发表于 2025-3-31 06:38:16
http://reply.papertrans.cn/59/5851/585006/585006_56.png察觉 发表于 2025-3-31 11:26:28
spp. Toolbox for Chemotaxis Assay,e methods allow to demonstrate chemotaxis defect, identify diverse chemoattractants, or decipher motile behavior quantitatively. These experiments have demonstrated a role of motility and potentially chemotaxis in leptospirosis pathogenesis. We describe extensively the methods and provide the key stOutmoded 发表于 2025-3-31 17:21:46
In Situ Structural Analysis of , spp. by Electron Cryotomography, are present inside the cell. Electron cryotomography (ECT) is an imaging technique that directly provides three-dimensional (3D) structures of cells and molecular complexes in their cellular environment at nanometer resolution. Here, I present a general protocol of ECT that covers the sample preparBlanch 发表于 2025-3-31 20:47:27
Measurement of the Cell-Body Rotation of ,y motors (flagellar motors) and transform the ends of the cell body into spiral or hook shape. The rotations of the periplasmic flagella are thought to gyrate the cell body and rotate the protoplasmic cylinder for propelling the cell; however, the motility mechanism has not been fully elucidated. Si去世 发表于 2025-3-31 22:45:15
In Vivo Imaging of Bioluminescent Leptospires, in vivo analysis. The use of pathogens, genetically modified to express with luminescent enzymes, combined to charge-coupled device (CCD) cameras, constitutes a major technological advance for assessing the course of infection in an intact, living host in real time and in a noninvasive way. This te