BOGUS 发表于 2025-3-28 16:09:29
Callum A. MacDonaldanalysis was first developed to study HIV-1 RNA genome packaging. In this assay, HIV-1 unspliced RNA is labeled with a fluorescently tagged RNA-binding protein (protein A) and some of the Gag proteins are labeled with a different fluorescent protein (protein B). Using fluorescence microscopy, HIV-1Accord 发表于 2025-3-28 21:39:40
Callum A. MacDonaldents require careful consideration of the fluorescent labeling method used and avoid or minimize its potential impact on normal viral replication and produce misleading results. Ideally, it is beneficial to utilize multiple virus labeling strategies and compare the results to ensure that the virionapiary 发表于 2025-3-29 01:16:26
Callum A. MacDonalduorescent virus particles, can be used to measure cell-to-cell transfer of virus particles. HIV NL-GI, a clone that expresses GFP as an early gene, facilitates the measure of productive infection after cell-to-cell contact. Lastly, a variation of the β-lactamase (BlaM)-Vpr fusion assay can be used t恸哭 发表于 2025-3-29 04:26:34
http://reply.papertrans.cn/55/5460/545917/545917_44.png驾驶 发表于 2025-3-29 11:04:29
Callum A. MacDonaldes for the inhibitor-induced aberrant IN multimerization, a dynamic light scattering-based assay which allows for monitoring the formation and sizes of oligomeric IN particles in a time-dependent manner, and a chemical cross-linking-based assay of interacting IN subunits which allows for the determiLANCE 发表于 2025-3-29 14:17:22
Callum A. MacDonalduorescent virus particles, can be used to measure cell-to-cell transfer of virus particles. HIV NL-GI, a clone that expresses GFP as an early gene, facilitates the measure of productive infection after cell-to-cell contact. Lastly, a variation of the β-lactamase (BlaM)-Vpr fusion assay can be used t幸福愉悦感 发表于 2025-3-29 15:54:06
http://reply.papertrans.cn/55/5460/545917/545917_47.png减震 发表于 2025-3-29 23:22:00
ked microbeads is employed. These methods allow for the isolation of highly purified virion preparations that are suitable for a wide variety of experiments, including the biochemical characterization of cellular proteins both on and in HIV virions, examination of virion/cell interactions, and imagiEndemic 发表于 2025-3-30 00:55:45
The Cold War and Counter-Revolutionthe right, placing conservatives in control of the security apparatus bequeathed by the defeated Japanese in an attempt to contain communism. Thus the North Korean attack was not merely an episode in the struggle amongst Koreans. On another level, it represented a clash between the Soviet and AmericGlutinous 发表于 2025-3-30 05:04:53
The Korean Decisionsccommodation with the Russians. Washington would have lost the cold war. The second was domestic. The new international climate had political repercussions at home, denting public confidence in containment. The Republicans, denied power in the Presidential elections of 1948, attempted to capitalize