triptans
发表于 2025-3-28 15:07:52
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爆炸
发表于 2025-3-28 20:20:50
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爆炸
发表于 2025-3-28 23:34:50
Progress in Targeting KRAS Directly,different types of cancer and the effects of these mutations on the biochemical, structural, and biophysical properties of the RAS proteins themselves, particularly KRAS, on which most attention has been focused. This knowledge base, while still growing, has enabled creative chemical approaches to t
Tortuous
发表于 2025-3-29 03:24:44
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争论
发表于 2025-3-29 08:07:59
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过份
发表于 2025-3-29 11:23:15
Nucleotide Exchange on RAS Proteins Using Hydrolysable and Non-hydrolysable Nucleotides, exchange the nucleotide present in the purified RAS protein with either GDPβS, GppNHp, or GTP depending on the assay requirement. In addition, we also describe the HPLC method used to validate the exchange process and provide information on the efficiency of the nucleotide exchange.
坚毅
发表于 2025-3-29 17:59:29
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ascend
发表于 2025-3-29 22:33:32
Considerations Around Structure-Based Drug Discovery for KRAS Using DOCK,ll molecules to cavities on the surfaces of proteins. Virtual screening for ligand discovery is a useful application of docking software. In this chapter, using the enigmatic KRAS protein as an example system, we endeavor to teach the reader about best practices for performing molecular docking with
嬉耍
发表于 2025-3-30 02:00:41
Measurement of KRAS-GTPase Activity, The development of biochemical assays for GTPase activity provides an opportunity to quantitatively measure the impact of these mutations on GTP hydrolysis. Here we describe a biochemical assay that measures the release of free phosphate upon hydrolysis of the GTP nucleotide and allows the measurem
constellation
发表于 2025-3-30 06:27:52
Affinity Measurement of Non-covalent Interactions of the Covalent KRAS G12C GDP Inhibitor MRTX849 ts label free and versatile, not limited to proteins, nucleic acids, and small molecules. SPR is a widely accepted method to measure not only affinity of molecular interactions but also association and dissociation rates of such interactions. In this chapter, we describe a general method to measure t