过多
发表于 2025-3-27 00:29:56
Tissue-Specific Transgenic and Knockout Miceciates with different β-subunits of the KCNE protein family. In the human heart, KCNQ1 associates with KCNE1 to generate the .. current characterized by its slow activation and deactivation kinetics. Mutations in either KCNQ1 or KCNE1 are responsible for at least four channelopathies that lead to ca
floodgate
发表于 2025-3-27 03:42:36
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白杨
发表于 2025-3-27 08:54:38
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大炮
发表于 2025-3-27 12:01:38
Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression Systemtate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na. channel heterologously expressed in Chinese hamster ovary cells.
一大群
发表于 2025-3-27 16:18:05
James D. Stockand,Mark S. ShapiroIncludes supplementary material:
conjunctiva
发表于 2025-3-27 18:58:12
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hemoglobin
发表于 2025-3-27 23:21:11
1064-3745 e their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy
赏钱
发表于 2025-3-28 04:24:59
Overexpression of Proteins in Neurons Using Replication-Deficient Virusiological techniques to assess the effects of overexpressing wild-type or mutant proteins in neurons. Here, a general protocol for the production of replicationdeficient Semliki Forest virus constructs directing the overexpression of proteins of interest in cultured mammalian neurons is described.
Tracheotomy
发表于 2025-3-28 09:14:01
Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using tensitive and blocker-insensitive subunits are coexpressed in a heterologous expression system. Details related to the expression of wild type and mutant ENaCs in . oocytes and the examination of blocker sensitivity by two-electrode voltage clamp, as well as analysis of data are provided.
SYN
发表于 2025-3-28 14:19:21
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