中世纪 发表于 2025-3-27 00:28:15

Dynamic and Static Light Scattering of Intrinsically Disordered Proteinsred proteins (IDPs), can be obtained from light scattering measurements. In this chapter, we discuss the physical basis of light scattering, experimental techniques, sample treatment, and data evaluation with special emphasis on studies on proteins. Static light scattering (SLS) is capable of measur

领导权 发表于 2025-3-27 02:55:20

Estimation of Intrinsically Disordered Protein Shape and Time-Averaged Apparent Hydration in Nativescattering) is a useful methodology to characterize hydrodynamic properties of macromolecules, including intrinsically disordered proteins. The time-averaged apparent hydration and the shape factor of proteins can be estimated from the measured parameters (molecular mass, intrinsic viscosity, hydro

CLASP 发表于 2025-3-27 06:33:52

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脱落 发表于 2025-3-27 12:04:16

Denaturant-Induced Conformational Transitions in Intrinsically Disordered Proteins also drive atypical responses of IDPs to changes in their environment. Among several specific features, the conformational behavior of IDPs is characterized by the low cooperativity (or the complete lack thereof) of the denaturant-induced unfolding. In fact, the denaturant-induced unfolding of nati

轻率的你 发表于 2025-3-27 16:52:53

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Malaise 发表于 2025-3-27 21:37:35

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Aggrandize 发表于 2025-3-28 00:16:15

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Accommodation 发表于 2025-3-28 02:05:41

Analyzing Temperature-Induced Transitions in Disordered Proteins by NMR Spectroscopy and Secondary Cn crucial for the understanding of the conformational properties of disordered proteins and is increasingly used to probe their conformational ensembles. Compared to folded proteins, disordered proteins are more malleable and more easily perturbed by environmental factors. Accordingly, the experimen

移动 发表于 2025-3-28 09:24:51

Application of Confocal Single-Molecule FRET to Intrinsically Disordered Proteinslecules. A particularly versatile method is highly sensitive fluorescence detection. In combination with Förster resonance energy transfer (FRET), distances and conformational dynamics can be investigated in single molecules. This chapter introduces the practical aspects of applying confocal single-molecule FRET experiments to the study of IDPs.

军械库 发表于 2025-3-28 12:56:06

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查看完整版本: Titlebook: Intrinsically Disordered Protein Analysis; Volume 2, Methods an Vladimir N. Uversky,A. Keith Dunker Book 2012 Springer Science+Business Med