Fortify
发表于 2025-3-28 17:02:07
Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerasebasic unit is a monomer repeat of approx 170 basepair (bp) that contains both sequences conserved among the different chromosomes and variable regions. Blocks of consecutive monomers comprise a chromosome-specific higher-order repeat of up to several kilobases (kb) in size. For most human chromosom
dry-eye
发表于 2025-3-28 19:42:19
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积习难改
发表于 2025-3-29 00:04:24
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Bmd955
发表于 2025-3-29 07:06:00
Primed In Situ (PRINS) Labeling of DNAdetection was possible, which to some extent made these techniques unsuited for routine purposes. Within the last decade, a number of nonradioactive detection methods have become available, resulting in a much more widespread use of . hybridization. These methods have become of great value, e.g., as
gentle
发表于 2025-3-29 08:28:01
Free Chromatin Mapping by FISHctrophoresis and blot-hybridization analysis, DNA sequencing, construction of somatic cell hybrid lines, . hybridization, as well as genetic (linkage) analysis. Because of their wide application, numerous improvements have been made in each of these areas in recent years, for example, the use of pul
戏服
发表于 2025-3-29 12:39:17
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固定某物
发表于 2025-3-29 16:12:53
In Situ Hybridization of Meiotic Prophase Chromosomesis in part, a result of traditional fixation procedures that use alcohol, acetic acid, and/or relatively strong ionic conditions, all of which can cause chromosome condensation. The limit of resolution may be improved by using free chromatin or DNA-halo preparations as described in . and . of this v
无弹性
发表于 2025-3-29 21:24:25
Strand-Specific Fluorescence In Situ Hybridization for Determining Orientation and Direction of DNA tation and strand direction of DNA sequences (.). Standard fluorescent . hybridization methods rely on nucleotide base pairing between a labeled probe and the complementary chromosomal target sequence. Both probe and target DNAs must be functionally single-stranded at the start of the procedure. Sin
惩罚
发表于 2025-3-30 01:30:45
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不可救药
发表于 2025-3-30 06:15:19
Gene Mapping Using 3H-Labeled Heterologous Probese cloned probe, labeled by a radioactive or fluorescent tag, hybridizes specifically to the single-stranded sequence(s) in the genome, and the location of the gene or sequence of interest on the chromosome is visualized by autoradiography or fluorography. Here we will discuss only radioactive . hybr