完全 发表于 2025-3-25 05:01:53
http://reply.papertrans.cn/47/4602/460142/460142_21.pngCOKE 发表于 2025-3-25 10:45:06
Anchal Garg,Jean-Paul Van Bellespecificities. Here, we describe a fast and simple protocol for small-scale packaging of CRISPR/Cas9 components into AAV vectors. To showcase its potential, we employ this method for screening of gRNAs targeting the murine miR-122 locus in Hepa1–6 cells (using AAV serotype 6, AAV6) or the 5′LTR of tAnalogy 发表于 2025-3-25 14:57:25
Lynn Futcher,Kerry-Lynn Thomson,Apelele Mbuqesage. We will also discuss the installation of a dockerized version of CRISPR Library Designer (CLD) that can be deployed locally or in the cloud to support the end-to-end design of sgRNA libraries for more than 50 different organisms. CLD was built upon E-CRISP to further increase the scope of sgRN提升 发表于 2025-3-25 16:17:13
http://reply.papertrans.cn/47/4602/460142/460142_24.png大约冬季 发表于 2025-3-25 23:22:30
Malie Zeeman,Romeo Botesequences, while the second provides a broad specificity profile based on cleavage of a massive library of potential off-target DNA sequences. Collectively, these assays may be used to evaluate the specificity of different CRISPR/Cas9 systems on any DNA target sequence in a time- and cost-effective mgorgeous 发表于 2025-3-26 03:36:21
http://reply.papertrans.cn/47/4602/460142/460142_26.pngRadiation 发表于 2025-3-26 07:49:39
http://reply.papertrans.cn/47/4602/460142/460142_27.png纬线 发表于 2025-3-26 10:01:51
http://reply.papertrans.cn/47/4602/460142/460142_28.png露天历史剧 发表于 2025-3-26 15:45:54
http://reply.papertrans.cn/47/4602/460142/460142_29.png胰岛素 发表于 2025-3-26 19:18:52
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