BLAZE
发表于 2025-3-26 22:37:53
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未完成
发表于 2025-3-27 01:24:31
Hypoxic Treatment of Zebrafish Embryos and Larvae,hod to induce hypoxia in zebrafish embryos and larvae. This protocol is easy and reproducible and does not require expensive equipment or specialized devices. It can be adapted in large, medium, and small scales. This protocol is also well-suited for experiments requiring chemical drug treatment and
侵害
发表于 2025-3-27 08:52:29
Microinjection of Antisense Morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into Zebrafish Embryos, capped mRNA, and DNA can be delivered into fertilized zebrafish eggs to manipulate gene expression during development. This protocol can also be adapted for microinjection in other fish and amphibian species.
contrast-medium
发表于 2025-3-27 13:26:06
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口味
发表于 2025-3-27 16:33:16
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candle
发表于 2025-3-27 20:36:28
https://doi.org/10.1057/978-1-349-94969-4ables us to understand the comprehensive regulation of gene expression by specific factors or environmental stimuli. Here, we describe the complete process of studying hypoxia-mediated gene expression by using RNA-Seq, including the hypoxic treatment of cells, RNA isolation, RNA quality check, cDNA library preparation, and library quality check.
过去分词
发表于 2025-3-28 01:57:36
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nitric-oxide
发表于 2025-3-28 06:02:19
Transcriptional Profiling Using RNA-Seq to Study Hypoxia-Mediated Gene Regulation,ables us to understand the comprehensive regulation of gene expression by specific factors or environmental stimuli. Here, we describe the complete process of studying hypoxia-mediated gene expression by using RNA-Seq, including the hypoxic treatment of cells, RNA isolation, RNA quality check, cDNA library preparation, and library quality check.
好色
发表于 2025-3-28 08:18:36
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有效
发表于 2025-3-28 12:17:09
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