EPT 发表于 2025-3-25 19:53:53
http://reply.papertrans.cn/44/4309/430807/430807_24.pngOmniscient 发表于 2025-3-25 23:10:48
Evaluating the Metabolic Impact of Hypoxia on Pancreatic Cancer Cells, alterations in NAD(P)H/NAD(P). and intracellular ATP levels, and increased utilization of glutamine as the major precursor for fatty acid synthesis. In this chapter, we describe in detail various physiological assays that have been adopted to study the metabolic shift propagated by exposure to hypoPainstaking 发表于 2025-3-26 03:07:47
http://reply.papertrans.cn/44/4309/430807/430807_26.png腐败 发表于 2025-3-26 08:01:14
http://reply.papertrans.cn/44/4309/430807/430807_27.pngdefenses 发表于 2025-3-26 09:52:23
http://reply.papertrans.cn/44/4309/430807/430807_28.png高兴去去 发表于 2025-3-26 16:36:07
http://reply.papertrans.cn/44/4309/430807/430807_29.pngConduit 发表于 2025-3-26 18:36:00
http://reply.papertrans.cn/44/4309/430807/430807_30.pngBLAZE 发表于 2025-3-26 22:37:53
http://reply.papertrans.cn/44/4309/430807/430807_31.png未完成 发表于 2025-3-27 01:24:31
Hypoxic Treatment of Zebrafish Embryos and Larvae,hod to induce hypoxia in zebrafish embryos and larvae. This protocol is easy and reproducible and does not require expensive equipment or specialized devices. It can be adapted in large, medium, and small scales. This protocol is also well-suited for experiments requiring chemical drug treatment and侵害 发表于 2025-3-27 08:52:29
Microinjection of Antisense Morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into Zebrafish Embryos, capped mRNA, and DNA can be delivered into fertilized zebrafish eggs to manipulate gene expression during development. This protocol can also be adapted for microinjection in other fish and amphibian species.