Halfhearted
发表于 2025-3-30 09:22:45
1064-3745 ation advice from the experts.This. .volume. .details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine. Chapters guide readers through .in vitro. systems, cell-based systems, comprehens
sundowning
发表于 2025-3-30 12:49:34
Book 2020f protein phosphorylation on histidine. Chapters guide readers through .in vitro. systems, cell-based systems, comprehensive background review articles on histidine kinases and phosphatases. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions
知识
发表于 2025-3-30 20:24:25
In Vitro Assays for Measuring Protein Histidine Phosphatase Activity,nd provide protocols for measuring the activity and inhibition of PHPT1 in vitro using the fluorescent probe 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP). This assay uses small quantities of commercially available materials, making its use feasible for most laboratories.
Default
发表于 2025-3-30 23:30:25
http://reply.papertrans.cn/43/4273/427225/427225_54.png
撕裂皮肉
发表于 2025-3-31 02:20:45
http://reply.papertrans.cn/43/4273/427225/427225_55.png
vitrectomy
发表于 2025-3-31 05:45:07
Proteome Bioinformatics Methods for Studying Histidine Phosphorylation,ard to run but require some theoretical knowledge to optimize parameters and correctly interpret outputs. We also describe some of the theory underpinning statistical considerations, to assist correct usage and interpretation of these bioinformatics methods.
温室
发表于 2025-3-31 11:15:51
http://reply.papertrans.cn/43/4273/427225/427225_57.png
易改变
发表于 2025-3-31 15:16:35
Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry,the absence and presence of a suitable phosphate donor, the stoichiometry of histidine phosphorylation can be determined. The described method can be readily adapted for the analysis of other proteins containing phosphohistidine.
ABOUT
发表于 2025-3-31 17:40:11
Manipulation of Bacterial Signaling Using Engineered Histidine Kinases,s related to bacterial cell survival, symbiosis, and virulence, and to develop sensory devices in synthetic biology. Here we focus on the principles for engineering the HK to identify unknown signal inputs, test signal transmission mechanisms, design small molecule sensors, and rewire two-component signaling networks.
CHIP
发表于 2025-3-31 22:40:00
Empirical Evidence of Cellular Histidine Phosphorylation by Immunoblotting Using pHis mAbs,an immunoblotting technique for detecting cellular phosphohistidine, a labile posttranslational modification, by optimizing experimental conditions such that the labile phosphohistidine signal is conserved throughout the experiment.