pulmonary 发表于 2025-3-28 17:05:16
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Protein Dynamics in Phosphoryl-Transfer Signaling Mediated by Two-Component Systems,ukaryotes. Such systems implicate a sensory histidine kinase and a separate response regulator protein. Both components exploit protein flexibility to effect specific conformational rearrangements, modulating protein–protein interactions, and ultimately transmitting information accurately. Recent wofiction 发表于 2025-3-29 16:43:46
A Quantitative Method for the Measurement of Protein Histidine Phosphorylation,ing for histidine kinase activity, allowing for the calculation of specific activity. In addition, quantitative time-course assays can also be performed to allow for kinetic analysis of histidine kinase activity.生来 发表于 2025-3-29 21:26:42
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http://reply.papertrans.cn/43/4273/427225/427225_49.png有抱负者 发表于 2025-3-30 04:57:11
High-Throughput Characterization of Histidine Phosphorylation Sites Using UPAX and Tandem Mass Spec), phosphothreonine (pThr), and phosphotyrosine (pTyr), are stable under these acidic binding conditions, phosphopeptides containing acid-labile phosphate group such as phosphohistidine (pHis), are not. Consequently, hydrolysis of these types of phosphopeptides occurs during standard phosphopeptide