大炮 发表于 2025-3-27 00:19:54
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GFP-LC3 High-Content Assay for Screening Autophagy Modulators also be used to identify autophagy modulators by screening a large number of compounds. This chapter describes a cell-based high content green fluorescent protein (GFP)-LC3 assay using mouse embryonic fibroblasts (MEF) stably expressing GFP-LC3.Anticonvulsants 发表于 2025-3-27 08:55:34
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Cell-Based Imaging Assay for Detection of Phospholipidosiscreening (HTS) platform. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a 1536-well plate, thus can be used for high-throughput screening (HTS) to identify chemical compounds that induce phospholipidosis.correspondent 发表于 2025-3-28 06:06:25
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Cell-Based Assays to Identify ERR and ERR/PGC Modulatorsgonists require examination of this nuclear receptor alone and together with PGC-1α. In this book chapter, we describe the step-by-step protocol of a multiplex luciferase assay designed to identify ERRα agonists, antagonists, and toxicity in one quantitative high-throughput screening assay using two different stable cell lines.欲望 发表于 2025-3-28 12:50:55
Cell-Based hERG Channel Inhibition Assay in High-Throughput Formattivity. This chapter describes a cell-based hERG channel inhibition assay that has been optimized and performed in a 1536-well plate format. The homogeneous and robust assay can be used to identify compounds that inhibit hERG channel activity.