绝种
发表于 2025-3-30 09:56:01
Wei Yuan,Chaojie Wu,Ning Zhou,Ke-Qin Zhangtection of single-copy genes in Southern blots and low abundance mRNAs in Northern blots. Labelling of (c)DNA probes involves replacement of dTTP by either digoxigenin-dUTP or biotinylated dUTP. Here we describe experimental protocols for Northern blot analysis and for genomic Southern blot using a
渐强
发表于 2025-3-30 15:17:53
Marzieh Parhizkar,Yan Zhao,Tong Lin the physical chemistry of DNA hybridization, at the end of the chapter we present a list of relevant references. In spite of a vast amount of literature on the subject the complex process of hybridization on solid support is still poorly understood. For this reasons many conditions used in this pro
有帮助
发表于 2025-3-30 18:50:52
Toshihiro Hirai,Hong Xiaer on hybridization, the stringency of the buffer can easily be increased by lowering the molarity of the monovalent cations and/or increasing the temperature. For all the genomic sequencing experiments carried out in our laboratory, the washing buffers described in the ‘Step-by-Step Procedure’ were
殖民地
发表于 2025-3-31 00:13:37
Mutsumi Kimurang gel to the nylon membrane. Many different parameters have to be taken into consideration: electrical resistance, strength of the electric field, diffusion, Joule heating, stability of the buffer, etc. Most of the transfer systems described in the literature so far (Towbin et al., 1979; Gibson, 19
frivolous
发表于 2025-3-31 04:09:39
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单片眼镜
发表于 2025-3-31 05:53:26
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incubus
发表于 2025-3-31 13:12:18
Tae Hwan Lim,Seong Hun Kim,Kyung Wha Ohgosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. 4 Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. 5 Measurement of Binding Constant for the Interaction Between Protein and ANTS-Labelled Car
Corroborate
发表于 2025-3-31 14:46:06
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Ringworm
发表于 2025-3-31 20:35:24
Lieva Van Langenhovecribed in this book. No trouble-shooting guide can ever replace solid judgement and a thorough understanding of the biological material at hand. Therefore, it would be unrealistic to hope to present an exhaustive list of all possible problems, causes and solutions. Nevertheless, I present here the m
GENUS
发表于 2025-4-1 00:07:45
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