宏伟 发表于 2025-3-30 08:16:04
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Circular mRNA Encoding for Monomeric and Polymeric Green Fluorescent Protein is produced through the splicing activity of autocatalytic group I RNA elements (.; .,.). Because the only cofactors required for splicing of the group I intron are magnesium and guanosine, the process can take place in a variety of organisms, making it amenable to a wide variety of protein expression systems (.–.).objection 发表于 2025-3-30 20:00:32
8 Fluorescence Resonance Energy Transfer (FRET) Applications Using Green Fluorescent Proteins. The point is stressed that, in most cases, FRET cannot be used to directly measure intermolecular distances, but only to estimate them. The reason for these uncertainties are made clear. First, the mechanisms of fluorescence and fluorescence energy transfer are briefly introduced.SYN 发表于 2025-3-30 21:29:14
Role of TRPM7 in Ischemic CNS Injury,hore of GFP is intrinsic to the polypeptide chain, and thus easily applied to molecular imaging in living cells (.). One of the useful methods for creating new molecular-recognition sites on GFPs is a combination of the insertional gene fusion technique with evolutionary biotechnology (.).Flawless 发表于 2025-3-31 01:26:21
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New Techniques for Future Accelerators III distinct forms, the far-red light-absorbing form (Pfr) and the red light-absorbing form (Pr). Of these two forms, Pfr is biologically active. Red light induces phototransformation of inactive Pr to active Pfr. Conversely, far-red light converts Pfr back to Pr. In this way, phytochrome acts as a molecular switch.